Curr Dev Nutr. 2019 Jun ;3(Suppl 1). Epub 2019 Jun 13. PMID: 31225172
Effects of Avocado Consumption on Gastrointestinal Microbial Metabolite Concentrations and Taxa Abundances: A Randomized, Controlled Trial (OR23-07-19).
Objectives: Avocados are rich in dietary fiber and monounsaturated fatty acids, nutrients that have been independently connected with metabolic health benefits and changes to the gastrointestinal (GI) microbiota. However, little is known regarding the impact of avocado consumption on GI microbial community composition and microbially-derived metabolites, particularly among adults with overweight or obesity.Methods: Adults ( = 160) between 25-45 years of age with BMI ≥ 25.0 kg/mwere enrolled in an investigator-blinded, parallel arm, randomized, controlled trial. Participants consumed isocaloric meals with or without fresh Hass avocado once daily for 12-weeks. Compliance was evaluated with daily self-report records. Fecal microbially-derived metabolites, including acetate, propionate, butyrate, isovalerate, valerate, and isobutyrate were quantified using gas chromatography mass spectroscopy on a dry matter basis. Following fecal DNA extraction, microbial analyses were conducted by sequencing the V4 region of the 16S rRNA gene. Sequence data were analyzed using DADA2 and QIIME version 2. Per protocol (PP;>80% meal consumption) and intent-to-treat (ITT) approaches were applied and generalized linear mixed models were assessed for treatment, time, and treatment by time interactions in SAS version 9.4.Results: The intervention was completed by 88% ( = 140) of participants, average meal compliance among both groups was 90%, and 83% ( = 132) of participants met PP criteria. Microbiota analyses were completed for 156 ITT and 109 PP participants, respectively. ITT time by treatment analyses indicated that compared to control, avocado consumption increased acetate (